1111. Removal of Empty Particles from Type 1 Adeno-Associated Virus Vector Stocks by Ion Exchange Chromatography Potentiates Transgene Expression

Disease-inducible transgene expression from a recombinant adeno-associated virus vector in a rat arthritis model.

Rheumatoid arthritis (RA) is a systemic autoimmune disease affecting 1% of the world's population, with significant morbidity and mortality. In this study, we investigated a recombinant adeno-associated virus (rAAV) vector for its potential application in RA gene therapy. rAAV encoding Escherichia coli beta-galactosidase was injected into rat joints which had already been induced into acute art...

Herpes simplex virus type 1/adeno-associated virus rep(+) hybrid amplicon vector improves the stability of transgene expression in human cells by site-specific integration.

Herpes simplex virus type 1 (HSV-1) amplicon vectors are promising gene delivery tools, but their utility in gene therapy has been impeded to some extent by their inability to achieve stable transgene expression. In this study, we examined the possibility of improving transduction stability in cultured human cells via site-specific genomic integration mediated by adeno-associated virus (AAV) Re...

Transgene Expression Transfer: Role of Cellular FKBP52 Protein in Adeno-Associated Virus Type 2-Mediated Gene

Removal of Aggregates of Monoclonal Antibodies by Ion Exchange Chromatography

Abstract—The primary objective of this work was to study the effect of resin chemistry, pH and molarity of binding and elution buffer on aggregate removal using Cation Exchange Chromatography and find the optimum conditions which can give efficient aggregate removal with minimum loss of yield. Four different resins were used for carrying out the experiments: Fractogel EMD SO3(S), Fractogel EMD ...

Enhancement of recombinant adeno-associated virus mediated transgene expression by targeted echo-contrast agent.

Ultrasound-targeted microbubble destruction (UTMD) has been recently developed for destroying bubbles carrying drugs or genes, thereby permitting local release of these target molecules. We investigated whether SonoVue®, a new contrast agent that contains phospholipid-stabilized microbubbles filled with sulfur hexafluoride vapor, is effective at delivering a recombinant adeno-associated viral (...